What are the steps involved in HPTLC?
What are the steps involved in HPTLC?
The HPTLC suite is composed of a number of components.
- Sample Applicator – Application of sample to stationary phase i.e. TLC/HPTLC plate.
- Development Chamber – Separation of components.
- Derivatizer set and Plate heater – Derivatization: Chemical spraying of plate to detect compounds not visible under white/UV light.
What is HPTLC technique?
High-performance thin-layer chromatography (HPTLC) is an enhanced form of thin-layer chromatography (TLC). A number of enhancements can be made to the basic method of thin-layer chromatography to automate the different steps, to increase the resolution achieved, and to allow more accurate quantitative measurements.
Which solvent is most widely used in HPTLC technique?
For reproducibility studies and quantitative analysis, layers are often prewashed using 20 ml methanol (generally, methanol is used as a prewashing solvent; however, a mixture of methanol and ethyl acetate or even mobile phase of the method may also be used) per trough in a 20 × 10 cm twin-trough chamber (TTC).
What is HPTLC in pharmacognosy?
Applications of High-Performance Thin Layer Chromatography (HPTLC): HPTLC technique is widely employed in the pharmaceutical industry in process development, identification, and detection of adulterants in herbal products and helps in the standardization of herbal drugs. HPTLC is an advanced sophisticated form of TLC.
Which lamps are used in HPTLC?
TLC Scanner III:With three light sources-mercury vapour lamp, deuterium lamp, or tungsten. HPTLC is a versatile separation technique and is official in the most of the pharmacopoeias for determining content uniformity, purity profile, assay values and dissolution rates in unlimited number of monographs.
Which is an instrument component in HPTLC?
30. Instrumentation of HPTLC consists of following: Lamp selector Entrance lens slit Monochromator entry slit Grating Mirror Slit aperture disc Mirror Beam splitter Reference photo multiplier Measuring photo multiplier Photo diode for transmission measurements.
Why nitrogen is used in HPTLC?
The most prominent advantage of using nitrogen as a carrier gas is that of all the carrier gases it is the most efficient when used at its optimum linear velocity (12 cm/s), meaning it will produce the narrowest chromatographic peaks.
Which software is used for HPTLC?
Software visionCATS
The visionCATS software organizes the workflow of the HPTLC analysis, controls the CAMAG instruments, and manages data evaluation. visionCATS stands for ease of use and intuitive simplicity.
What are the applications of HPTLC?
HPTLC is used for purity control of chemicals, pesticides, steroids, and water analysis. [50] HPTLC is also widely used for analysis of vitamins, water-soluble food dyes, pesticides in fruits, vegetables, and other food stuffs.
What is HPLC calibration parameters?
Standard Operating Procedure (SOP) and Format/Protocol for Calibration of HPLC system (Parameters are Pressure test, Drift and Noise, Flow Rate Accuracy, Autosampler by carryover check, detector by linearity measurement, etc.)