What is cool on column?

Cool On-Column This inlet, as the name implies, is cooled during injection, and allows sample to be injected directly onto the column.

What is inlet in GC?

In gas chromatography (GC), the inlet is the first part of the instrument with which your sample can interact. The inlet’s main purpose is to transfer the sample onto the GC column for analysis.

What is an Agilent column?

Small Molecule Columns Agilent offers one of the broadest, most modern liquid chromatography (LC) column portfolios for small molecule analysis, with columns suitable for nearly all separation modes and applications.

What is on column injection?

On-column injection employs the direct introduction of the liquid sample into the column. This is very useful for the analysis of thermally unstable samples and avoids boiling point discrimination.

Why is on column injection used?

The On-Column Injector A mode of injection that avoids the hot injection liner all together is useful for thermally unstable (labile) or for GC samples with large, analyte-boiling-point differences that suffer discrimination in flash vaporization. This is the cold on-column injector.

What is on column injection in GC?

On-Column Injection in Capillary Gas Chromatography (COC) without press fits. With on-column injection a liquid sample is introduced directly into the column with a thin injection needle. During the course of the temperature program the vapour pressure of the solutes increases and the chromatographic process begins.

How do you bake out GC column Agilent?

Troubleshooting Agilent 5975 and 5977 GC/MS Air Leaks and resolve the air leak.

1. Click Instrument > GC Parameters > Columns – set the Gas Chromatograph column flow to 1.2 ml/min then Apply and Exit back to the main menu.
2. Click View > Tune and Vacuum Control.
3. Click Execute> Bake out MSD and then set the following parameters.